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Viral RNA and aerosol particles in respiratory emissions. The number of viral gene copies in aerosol expulsions was determined using droplet digital PCR during ( A ) breathing and ( B ) speaking. Aerosol particle concentrations measured during ( C ) breathing and ( D ) speaking. Measured particle concentrations (for particles of 0.3–25 µm size range) were normalized to background particle concentrations in the corresponding participant’s room. The dashed horizontal line indicates a ratio of 1 with respect to the background.

Journal: Journal of Virology

Article Title: Influenza virus infection and aerosol shedding kinetics in a controlled human infection model

doi: 10.1128/jvi.01612-24

Figure Lengend Snippet: Viral RNA and aerosol particles in respiratory emissions. The number of viral gene copies in aerosol expulsions was determined using droplet digital PCR during ( A ) breathing and ( B ) speaking. Aerosol particle concentrations measured during ( C ) breathing and ( D ) speaking. Measured particle concentrations (for particles of 0.3–25 µm size range) were normalized to background particle concentrations in the corresponding participant’s room. The dashed horizontal line indicates a ratio of 1 with respect to the background.

Article Snippet: NP swab quantitative PCR assessment: NP swabs collected on the day of admission were used to ensure participant eligibility with a negative multiplex upper respiratory pathogen panel (BioFire, Salt Lake City, UT).

Techniques: Aerosol, Digital PCR

Viral RNA and aerosol particles in respiratory emissions. The number of viral gene copies in aerosol expulsions was determined using droplet digital PCR during ( A ) breathing and ( B ) speaking. Aerosol particle concentrations measured during ( C ) breathing and ( D ) speaking. Measured particle concentrations (for particles of 0.3–25 µm size range) were normalized to background particle concentrations in the corresponding participant’s room. The dashed horizontal line indicates a ratio of 1 with respect to the background.

Journal: Journal of Virology

Article Title: Influenza virus infection and aerosol shedding kinetics in a controlled human infection model

doi: 10.1128/jvi.01612-24

Figure Lengend Snippet: Viral RNA and aerosol particles in respiratory emissions. The number of viral gene copies in aerosol expulsions was determined using droplet digital PCR during ( A ) breathing and ( B ) speaking. Aerosol particle concentrations measured during ( C ) breathing and ( D ) speaking. Measured particle concentrations (for particles of 0.3–25 µm size range) were normalized to background particle concentrations in the corresponding participant’s room. The dashed horizontal line indicates a ratio of 1 with respect to the background.

Article Snippet: Screening involved medical history, physical examination, laboratory, electrocardiogram (ECG), a negative multiplex upper respiratory pathogen panel (BioFire, Salt Lake City, UT), and chest radiograph imaging.

Techniques: Aerosol, Digital PCR